The xponent application is licensed in trial mode for 30 days. The elx50 microplate strip washer biotek instruments used in these experiments was configured with a 96well flat magnet pn 7103016. Designed for easeofuse, xponent allows all types of usersnovice, occasional, and advancedto set up and run an assay in a minimal amount of time. Under no circumstances should you remove the luminex 200 analyzer cover. B april 2011 luminex corporation luminex reserves the right to modify its products and services at any time. The target value files, or lxl file, can also be downloaded and saved to your luminex 100200 system. The first is xmap microspheres, a family of fluorescently dyed micronsized polystyrene microspheres that act as both the identifier and the solid surface to build the assay.
Page 8 when you turn on the luminex magpix system, the following start up indicators occur. This tab allows the user to restore a specific page layout to its default settings. The bioplex 3d suspension array system is the next generation multiplexing platform based on xmap technology. We will also continue to provide service, support, accessories, and software for customers with the bioplex magpix multiplex reader. Backing up the bioplex manager mp database it is a best practice to back up the database frequently and keep the backup copies in a safe location. Manage luminex systems the user may enter multiple luminex instruments when in the manage luminex systems window. The labscan3d and labscan 100 systems are based on luminex xmap technology and use luminex xponent software for data acquisition. Enduser license agreement eula for luminex software this luminex enduser license agreement eula is a legal agreement between you either an individual or a single entity, also referred herein as you the enduser and luminex corporation luminex regarding the use of the xponent software product provided to you above. The procedures are presented as stepbystep instructions and assume basic knowledge of xponent software and xmap technology.
The ability to use either magnetic or polystyrene beads makes this instrument a popular choice for laboratories needing greater flexibility. This luminex enduser license agreement eula is a legal agreement between you either an individual or a single entity, also referred herein as you the enduser and luminex corporation luminex regarding the use of the xponent software product. For detailed instructions refer to the instrument user manual or the luminex xponent software user manual. Unknowns can be fit to the standard curve by the xponent software allowing relative quantitation. Luminex multiplexing technology consists of beadbased immunoassays using xmap beads, high plex multiplexing instrument, and powerful software. Intended use perform multiple assays with a single sample with the athena multilyte test analyzer, built by luminex corporation on the luminex 100200 system using xmap technology. When the software is first installed, it will search the network for existing luminex databases. Ifuen rev k intended use pak lx assay is a qualitative immunoassay for use on the luminex 100 or 200 instrument. Additionally, please refer to our luminex assays support center for data analysis tips and tricks. Select four vials of low concentration magplex microsphere bead sets or regions, for example, luminex part numbers mc10012id, mc100id, mc10014id, and mc10015id. Luminex 200 system user manual the following label appears on the back of the luminex 200 instrument. Only certain upgraded software versions on the luminex 100 instrument are compatible with the magnetic kit. The data present in the csv file must be generated using an unmodified luminex template that is provided by immucor gti diagnostics, inc. Luminex multiplexing technology consists of beadbased immunoassays using xmap beads, high.
Download and view lx200 instrument and software quick guide for routine setup instructions. Chemokine, and growth factor assays instruction manual for technical support, call your local biorad office, or in the u. Browse luminex accessories and learn about our luminex software, luminex beads compactivity and more. It provides details about the use of xponent software, different versions of xponent software, and the various functions that can be performed on xmap instruments from xponent software. Original mode 1 2 3 4 5 6 7 8 9 10 11 12 14 15 16 17 18 19. If your luminex instrument needs attention or you require. Luminex 200 system user ivd, portuguesebrazil manual, xponent 4.
Torq xponent user guide 3 introduction 1 thank you for choosing the maudio xponent usb dj controller and audio interface. Figures 2 and 3 show the standard curves for each analyte from the magpix and luminex 200 analysis respectively. All luminex instruments come with xponent data analysis software that can be used. Posted on august 7, 2014 november 24, 2015 by luminex corporation. Luminex lx200 with xponent software zeus scientific. Fast read time, compatibility with 96 and 384well plates, and ease of integration with frontend automation systems make the flexmap 3d the. Luminex learning field service engineers sessions luminex. This multiplex, fluorescent, beadbased system offers a simple and efficient alternative to ifa and elisa testing. Once the xponent drivers have been installed on your computer, torq should automatically detect the controller and allow you to use the buttons, knobs, sliders, and wheels without. The second is a flow cytometrybased instrument, the luminex 100200 analyzer, which. Enduser license agreement eula for luminex software. Luminex logo home link clinical infectious disease coronavirus covid19 respiratory infection bloodstream infection.
Jan 15, 2018 heres how to start up the luminex 200 instrument 1. Turn on the luminex 200 analyzer, xy platform, and, if used, the luminex sheath delivery system. Luminex xmap technology life science research milliporesigma. Systems include the luminex magpix, luminex 200, and luminex flexmap 3d. Start the luminex xponent program from the start menu or the desktop shortcut. This manual provides stepbystep instructions on how to start your magpix system for the first time, how to perform daily operations, and how to properly maintain the instrument. The luminex magpix instrument system is the simplest, most affordable, and most compact luminex instrument. Bioplex pro cytokine, chemokine, and growth factor assays. To create a new protocol name youll need to complete the following information. Serial number, luminex id, and luminex server should be entered and then saved. Its selfcleaning routines and magnetic bead compatibility with magplex beads make magpix extremely easy to learn and use. This innovative device gives digital djs all the familiar knobs, sliders, and wheels found on a dj mixer and cd players while allowing users to. Nxtag cov extended panel assay package insert for in vitro diagnostic use. This information is presented to support researchers who use magpix, 100200, and flexmap 3d luminex systems.
We will continue to provide premium luminex instrumentation. This imagebased multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96wellplate in just 60 minutes. Stenograph luminex user manual pdf download manualslib. Luminex assumes no liability for errors or omissions, or for any damages resulting from the. System startup verify that the following procedures were performed when the magpix system was turned on for the first time.
Xponent for magpix read online or download pdf luminex xponent for magpix user manual. Torq xponent user guide using xponent using xponent with torq xponent ships with syncroscience torq, a powerful dj application that works with xponent out of the box. Hardware user manual that you received with your instrument. A january 2007 luminex corporation luminex reserves the right to m odify its products and services at any time. User customizationthe default graphical user interface follows a typical assay workflow, and it can be customized to meet the needs of individual laboratories. Although prepared to ensure accuracy, luminex assumes no liability for errors or omissions, or for any damages. Although prepared to ensure accuracy, luminex assumes no liability for. This innovative device gives digital djs all the familiar knobs, sliders, and wheels found on a dj mixer and cd players while allowing users to harness the power of the latest dj software. The monkey cytokine magnetic 29plex panel is designed for the quantitative determination of egf, eotaxin, fgfbasic, gcsf, gmcsf, hgf. Designed for easeofuse, xponent enables both new and advanced users to set up and run assays in a minimal amount of time. Using the labscan systems with our labtype and labscreen family of products allows the user to make assignments of hla antibodies as well as hla typing with the analysis support of hla fusion.
Whenever a link fails or breaks, the redundant link automatically takes over the signal in just milliseconds. Luminex technology employs microsphere beads, either polystyrene or paramagnetic, that are colorcoded. Whether you are testing for single or multiple analytes, our magpix system and qualified assays offer accurate analytical performance and efficient, easy. The bioplex magpix multiplex reader is now discontinued. Please contact luminex technical support to obtain a permanent license. Designed for easeofuse, the software allows the novice or occasional user and advanced user alike, to set up and run an assay in a minimal amount of time. This system is a flexible analyzer based on the principles of flow cytometry which integrates key xmap detection components to meet the multiplex testing needs of clinical and research laboratory professionals. Luminex 200 optical alignment video online training this video provides information about luminex 200 updates for lblock alignment laser safety online training this 15 minute course shows the user information on laser safety in a selfpaced and interactive online setting where the enduser receives important information through audio and visual aids.
Luminex xmap technology, the basis for our milliplex map assay kits, is a powerful platform for multiplex detection of proteins in a single biological sample. Samples were then read on either a luminex magpix or a lx100 reader with xponent software using the parameters outlined in the assay kit instructions. Luminex corporation 12212 technology boulevard austin, texas 787276115 u. This protocol is a stepbystep method for setting up the xponent 3. Luminex 200 system with xponent software from thermo fisher. The pak lx assay is designed to detect and differentiate between igg antibodies to hpa1, hpa2, hpa3, hpa4, hpa5, gpiv and class i hla in human serum. Luminex 200 system user manual pdf download manualslib. Luminex corporation luminex regarding the use of the xponent. It is also necessary to select a date format used in the luminex system which specifies the run date format in the output files generated by luminex. Bioplex 3d suspension array system life science research.
Magpix system how to use protocol and batch function youtube. Luminex, magpix, and xponent are trademarks of luminex corporation. When performing routine maintenance, turn power to the luminex 200 analyzer off and disconnect the power cord. Like the luminex 200 and flexmap 3d systems, the magpix system uses xponent software, offering performance and user interaction similar to these more expensive flow cytometrybased systems. If the trial period expires, the application will become inoperable. Contact luminex technical support by telephone in the u. Also on power and temperature level our switches offer redundancy. Antibody coupling kit user manual pn 890000200319 rev. Expanded multiplexing capability, faster time to results, and automation capability make it the platform of choice for highthroughput testing for nucleic acid and protein applications. With luminex switches and the rlinkx protocol you dont have to worry about losing signal during a big show. The software should be fully licensed or uninstalled before the trial period expires.
On the pc desktop, click the luminex xponent icon, or click start all programs. Luminex xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Oct 12, 2012 to start, open up the protocol tab in xponent and select create new protocol. The luminex instrument to which the batch is to be.
The luminex 200 system is a compact analyzer that performs up to 100 assays simultaneously in a single well of a microtiter plate. If you need technical support, you can contact us 24 hours a day by email, call 18777852323, or visit our customer center for more local help. Luminex 200 system user manual pn 890000200173 rev. The xponent software package is recommended for data analysis. When performing routine maintenance, turn power to the luminex 200 analyzer off and. Luminex 200 system user manual university of sydney. Dna software is designed to import csv files created by the luminex xponent software. See the xponent license agreement for more information. Laser safety online training this 15 minute course shows the user information on laser safety in a selfpaced and interactive online setting where the end. Posted on august 7, 2014 november 24, 2015 by luminex.
1163 1208 224 1017 1467 1135 811 9 1226 1524 191 1372 1010 624 1578 1 958 1568 993 203 449 1503 841 575 1530 112 751 70 1498 669 468 174 707 831 310 187 1392 239 1032 783 877 335 1171